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Figure S2. Analysis of human ES cells for markers of pluripotency.
Human embryonic stem cells were analyzed by immunohistochemistry for the characteristic pluripotency markers Oct4 and SSEA-3. For reference, nuclei were stained with DAPI. Our analysis indicated that >90% of the ES cell colonies were positive for Oct4 and SSEA-3. Alkaline phosphatase activity was also strongly detected in human ES cells.