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A Chromatin Landmark and Transcription Initiation at Most Promoters in Human Cells

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Array Design

Whole Genome Array
The human genome array was purchased from Agilent Technology (www.agilent.com). The array consists of 25 slides each containing ~244,000 60mer oligos (slide ID 14818-14841 and 14843) covering the entire non-repeated portion of the human genome at a density of about 1 oligo per 250bp.
185K Promoter Array
The human promoter array was purchased from Agilent Technology (www.agilent.com). The array consists of 2 slides each containing ~185,000 60mer oligos (slide ID 14154, 14155) designed to cover regions between approximately -5.5 kb and +2.5 kb relative to the transcription start sites of ~17,350 genes at a density of about 1 oligo per 250 bp.
44k Promoter Array
The human promoter array was purchased from Agilent Technology (www.agilent.com). The array consists of 10 slides each containing ~44,000 60mer oligos designed to cover regions between approximately -8kb and +2kb relative to the transcription start site. The design of these arrays are discussed in detail elsewhere (Boyer et al., 2005).

 
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