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Figure 3. Enrichment of H3K9,14Ac and Pol II at inactive genes
A-B. Enrichment ratios are shown for histone H3 lysine-9,14 acetyl (H3K9,14Ac), which is associated with initiation. The start and direction of transcription are noted by arrow.
C. Composite enrichment profiles for H3K9,14Ac at genes called present or absent for mRNA transcripts using microarray data (as in Figure 2G).
D. Single probe enrichment ratios of H3K9,14Ac across the average 2.5kb region surrounding the transcription start site (~10 probes per promoter region). Enriched probes (blue) and unenriched probes (white) are shown for each gene. Individual promoters are represented top to bottom and ordered by average binding ratio within 1kb surrounding transcription start site (as in Figure 1D).
E. H3K9,14Ac maximum enrichment (blue horizontal line) within 1kb of the transcription start site for each gene represented on human promoter array. Genes are ordered as in (D).
F-G. Enrichment ratios are shown for initiating Pol II (antibody 8WG16).
H. Composite enrichment profiles for initiating Pol II (antibody 8WG16) at genes called present or absent for mRNA transcripts using microarray data (as in Figure 2G).
I. Single probe enrichment ratios of initiating Pol II (antibody 8WG16) across the average 2.5kb region surrounding the transcription start site (10 probes per promoter). Enriched probes (blue) and unenriched probes (white) are shown for each gene. Individual promoters are represented top to bottom and ordered by average binding ratio within 1kb surrounding transcription start site (as in Figure 1D).
J. Initiating Pol II (8WG16) maximum enrichment (blue horizontal line) within 1kb of the transcription start site for each gene represented on human promoter array. Genes are ordered as in (I).