Activated Signal Transduction Kinases Frequently Occupy Target Genes

Study Design

• Reagents
• Technology
• Location Analysis Protocols
• Data Processing
• Normalization and Analysis
• Identification of Bound Regions
• Error Rates
• Controls

• Download Location Data
• Data Analysis

• MAPK
  • Hog1
  • Fus3 and Kss1
  • Slt2 and Smk1
• Protein Kinase A
  • Tpk 1
  • Tpk 2

• Supplemental Figures
• Supplemental Tables

Having detected binding for three MAPKs in yeast, we attempted to determine whether the remaining two MAPKs in yeast, Slt2 and Smk1, also occupy the genes they ultimately regulate.

The Slt2p MAPK is a component of the cell integrity pathway and is active during the cell cycle, during mating and in response to environmental conditions that affect the cell wall. We did not detect association of Slt2p with the genome in ChIP-Chip experiments conducted under several conditions that activate this pathway, so it is possible that this MAPK does not associate with genes that are regulated in response to MAPK activation.