Activated Signal Transduction Kinases Frequently Occupy Target Genes

Study Design

• Reagents
• Technology
• Location Analysis Protocols
• Data Processing
• Normalization and Analysis
• Identification of Bound Regions
• Error Rates
• Controls

• Download Location Data
• Data Analysis

• MAPK
  • Hog1
  • Fus3 and Kss1
  • Slt2 and Smk1
• Protein Kinase A
  • Tpk 1
  • Tpk 2

• Supplemental Figures
• Supplemental Tables

The observation that at least 3 of the 5 yeast MAPKs occupy their target genes upon activation of their cognate signaling pathways led us to investigate whether kinases associated with another type of signaling pathway, the cAMP-activated protein kinase A (PKA) pathway, exhibit similar behavior. In yeast, the 3 catalytic subunits Tpk1p, Tpk2p and Tpk3p are stimulated by increases in cAMP levels when fermentable carbon sources such as glucose are introduced (Thevelein and de Winde 1999; Schneper et al. 2004). (Fig. 3A).