Activated Signal Transduction Kinases Frequently Occupy Target Genes

Study Design

• Reagents
• Technology
• Location Analysis Protocols
• Data Processing
• Normalization and Analysis
• Identification of Bound Regions
• Error Rates
• Controls

• Download Location Data
• Data Analysis

• MAPK
  • Hog1
  • Fus3 and Kss1
  • Slt2 and Smk1
• Protein Kinase A
  • Tpk 1
  • Tpk 2

• Supplemental Figures
• Supplemental Tables

As far as possible, we tested genetically whether the TAP-tagged proteins perform the function of the wild-type protein and did not find evidence that the TAP interfered with the function of the tagged proteins. We found that strains with TAP-tagged Fus3p, Kss1p and Ste5p form mating projections in the same manner as wild-type strains. In strains with TAP-tagged Tpk1p, we deleted both tpk2 and tpk3 and found that the strain is still viable. Similarly, the TAP-tagged Tpk2p strain in which we deleted both tpk1 and tpk3 is viable. It has previously been shown that at least one functional copy of a tpk gene is required for viability.