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Control of Developmental Regulators by Polycomb in Human Embryonic Stem Cells

Mapping RNA Polymerase II Occupancy in Embryonic Stem Cells

Data Global Transcriptional Repression by PRC2
Key Developmental Regulators Are Targets of PRC2
PRC2 and Highly Conserved Elements
Signaling Genes Are Among PRC2 Targets
Activation of PRC2 Target Genes During Differentiation
Supplementary Information




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Technology and Protocols

DNA sequences occupied by the initiation form of RNA polymerase II or by the PCR2 component Suz12 were enriched using chromatin-immunoprecipitation (ChIP) and identified with a set of 115 DNA microarrays containing over 4.6 million unique 60-mer oligonucleotide probes spanning the entire non-repeat portion of the human genome. This webpage describes the ChIP-chip protocol and microarray technology used in this experiment.

Figure 1a. Genome-wide ChIP-Chip in human embryonic stem cells. DNA segments bound by the initiation form of RNA polymerase II or Suz12 were isolated using chromatin-immunoprecipitation (ChIP) and identified with DNA microarrays containing over 4.6 million unique 60-mer oligonucleotide probes spanning the entire non-repeat portion of the human genome.

Antibodies
Chromatin Immunoprecipitations
Microarray Design
 
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