Control of Developmental Regulators by Polycomb in Human Embryonic Stem Cells

Mapping RNA Polymerase II Occupancy in Embryonic Stem Cells

• Human ES Cells
• Growth Conditions
• Immunohistochemical Analysis of Pluripotency Markers
• Teratoma Formation
• Embryoid Bodies (EB)
• Technology and Protocols
• Antibodies
• Chromatin Immunoprecipitations
• Microarray Design

• Download Data
• Data Analysis

DNA sequences occupied by the initiation form of RNA polymerase II or by the PCR2 component Suz12 were enriched using chromatin-immunoprecipitation (ChIP) and identified with a set of 115 DNA microarrays containing over 4.6 million unique 60-mer oligonucleotide probes spanning the entire non-repeat portion of the human genome. This webpage describes the ChIP-chip protocol and microarray technology used in this experiment.

Figure 1a. Genome-wide ChIP-Chip in human embryonic stem cells. DNA segments bound by the initiation form of RNA polymerase II or Suz12 were isolated using chromatin-immunoprecipitation (ChIP) and identified with DNA microarrays containing over 4.6 million unique 60-mer oligonucleotide probes spanning the entire non-repeat portion of the human genome.