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Culture and Quality Control of Human ES Cells
H9 human ES cells were cultured as recommended by the WiCell Institute
(www.wicell.org). We also tested them by immunohistochemistry for
characteristic stem cell markers, their ability to generate cell types
from all three germ layers upon differentiation into embryoid bodies and
their ability form teratomas in immunocompromised mice.
Growth Conditions
Immunohistochemical Analysis of Pluripotency Markers
Teratoma Formation
Embryoid Bodies (EB)
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