Control of Developmental Regulators by Polycomb in Human Embryonic Stem Cells

Mapping RNA Polymerase II Occupancy in Embryonic Stem Cells

• Human ES Cells
• Growth Conditions
• Immunohistochemical Analysis of Pluripotency Markers
• Teratoma Formation
• Embryoid Bodies (EB)
• Technology and Protocols
• Antibodies
• Chromatin Immunoprecipitations
• Microarray Design

• Download Data
• Data Analysis

We mapped the location of both RNA polymerase II and the Suz12 subunit of PRC2 genome-wide in human ES cells. Bound DNA sequences were identified in chromatin-immunoprecipitation (ChIP) experiments using DNA microarrays that contain over 4.6 million unique 60-mer oligonucleotide probes spanning the entire non-repeat portion of the human genome.

This web page provides links to further information describing the ES cell culture conditions, experimental protocols and microarray design.