Global Nucleosome Occupancy
In order to map nucleosome occupancy throughout the yeast genome, we carried
out immunoprecipitation with antibodies against core histone H3 or histone H4.
Histone occupancy at a region around the CPA1 gene is shown at left.
A composite of 5,324 genes is shown in the average gene displayed at right.
In general, occupancy appears to be reduced within intergenic regions relative
In agreement with previous gene-specific and
genome-wide studies, we find that occupancy by nucleosomes is inversely
correlated to transcripional
activity (H3 occupancy is shown at left).