Global Nucleosome Occupancy

In order to map nucleosome occupancy throughout the yeast genome, we carried out immunoprecipitation with antibodies against core histone H3 or histone H4. Histone occupancy at a region around the CPA1 gene is shown at left. A composite of 5,324 genes is shown in the average gene displayed at right. In general, occupancy appears to be reduced within intergenic regions relative to ORFs.