Study Design

Genetic Reagents Technology Protocols Analysis

Location Analysis

Search GAL4 location data Search STE12 location data Download datasets

Gene Expression Data

Galactose Pheromone Response

Additional Insights

General Insights GAL4 STE12

Our long term goal is to understand how DNA-binding proteins control global gene expression, chromosomal replication and cellular proliferation in vivo. We have developed a genome-wide location profiling method for DNA-bound proteins and have used it to monitor dynamic binding of Gal4 and Ste12, two gene-specific transcription activators of yeast cells. Genome-wide location analysis should be a powerful tool for further dissecting gene regulatory networks, annotating gene functions and exploring how genomes are replicated.

Schematic summary of the Genome-wide location profiling method.
The genome-wide location analysis method we have developed allows protein-DNA interactions to be monitored across the entire yeast genome. The method combines a modified Chromatin Immunoprecipitation (ChIP) procedure, which has been previously used to study in vivo protein-DNA interactions at one or a small number of specific DNA sites (Aparicio, 1999; Orlando et al., 2000), with DNA microarray analysis.