Genome-wide Expression

Study Design

• Project Goals
• Genetic Reagents
• Technology, Protocols, and Analysis
  • Harvesting Cells
  • Total RNA preparation
  • mRNA preparation
  • Controls
  • Reproducibility
  • Genes Scored
  • Fold Change
  • Genome Dependence
  • Assessment of Primary/Secondary Effects

Yeast mRNA Population

• Transcriptome
• Apparent Half-life and Transcriptional Frequency

Genome-Wide Expression Data

• RNA Polymerase II
• SRB/Mediator Core Complex
• SRB10 CDK Complex
• SWI/SNF Complex
• General Transcription Factors
• SAGA
• Search for component:
• Search for gene:

Results and Insights

• Summary
• Table of Results