Study Design

Genetic Reagents Technology Location Analysis Protocols Analysis

Location Analysis

Quality Control Search for Activator Binding Sites Download Datasets Table of Regulated Genes Previous Evidence of Regulation

Gene Expression Data

Alpha Factor Synchronization

Insights

Cell Cycle Regulation Additional Insights Summary

Genomic binding sites were identified for the nine known yeast cell cycle transcription activators, revealing how these factors coordinately regulate global gene expression and diverse stage-specific functions to produce a continuous cycle of events. One fundamental insight that emerged from these results is that a complete transcriptional regulatory circuit is formed by activator complexes that control next-stage activators. The results also show that stage-specific activator complexes regulate genes encoding CDK regulators necessary for both stage entry and for progression into the next stage of the cell cycle. This global information forms the foundation for a map of the regulatory network that controls the cell cycle.

Cell cycle and selected regulators

The stages of the cell cycle are depicted together with yeast cell morphology (yellow), transcriptional regulators (blue), and the cyclins that regulate Cdc28 activity (green). The transcriptional regulators and cyclins are positioned to represent the stage during which they are thought to function (Mendenhall and Hodge, 1998; Breeden, 2000).